Protein Coimmunoprecipitation in Arabidopsis - CSH Protocols
网页Coimmunoprecipitation is one of the most useful techniques for revealing protein-protein interactions. Briefly, it can be divided into three steps: (1) preparation of cell extracts without disruption of protein interactions; (2) formation of the antibody-antigen complexes; and (3) affinity purification of the protein complexes and subsequent ...
Coimmunoprecipitation (co-IP) of Nuclear Proteins and ... - PubMed
网页2008年9月1日 · Coimmunoprecipitation (co-IP) is a straightforward technique to study in vivo protein-protein interactions, and can identify interacting proteins or protein complexes present in cell extracts.
PAWH1 and PAWH2 are plant-specific components of an Arabidopsis …
网页2019年8月2日 · Here we report two closely-related Arabidopsis proteins, Protein Associated With Hrd1-1 (PAWH1) and PAWH2, which share a conserved domain with yeast Altered Inheritance of Mitochondria24.
The cytosolic thiol peroxidase PRXIIB is an intracellular sensor for ...
网页2022年10月14日 · This article shows how an Arabidopsis thiol peroxidase, PRXIIB, senses endogenous H2O2 and regulates immune responses through a redox relay mechanism.
Co-Immunoprecipitation of Membrane-Bound Receptors - PMC
网页2015年6月3日 · This Co-IP protocol has been developed to test the interaction of receptor-like kinases, their interacting partners, and peptide ligands in stable Arabidopsis thaliana lines, but can be modified to test interactions in transiently expressed proteins in tobacco, and potentially in other plant models, or scaled for large-scale protein-protein ...
Regulation of Arabidopsis photoreceptor CRY2 by two distinct E3 ...
网页2021年4月12日 · CRY2/LRB complexes are also detected by the co-IP (co-immunoprecipitation) assays in transgenic Arabidopsis seedlings stably co-expressing Flag-GFP-tagged CRY2 (FGFP-CRY2) and Myc-LRB1 or FGFP...
Simple Nuclei Preparation and Co-immunoprecipitation …
网页Here we describe a relatively simple and yet clear nuclei isolation method using Arabidopsis plants [23, 24]. Isolated nuclei samples can be utilized for western blot analysis as well as other assays to analyze protein-protein interactions (which we …
Co-immunoprecipitation Assay for Blue Light-Dependent …
网页Here we describe the protocol for detecting blue light-dependent protein interactions, particularly for blue light receptor cryptochrome-mediated complex formation. In addition, we present some notes which may be helpful for common Co-IP study as well. Keywords: Arabidopsis; CRY; CoIP; Inducible interactions.
Coimmunoprecipitation (co-IP) of Nuclear Proteins and Chromatin ...
网页2008年12月3日 · Coimmunoprecipitation (co-IP) is a straightforward technique to study in vivo protein-protein interactions, and can identify interacting proteins or protein complexes present in cell extracts.
Coimmunoprecipitation (co-IP) of Nuclear Proteins and Chromatin ...
网页2008年9月1日 · Detailed protocols for extraction of Arabidopsis seedlings, co-IP of nuclear proteins, and Chromatin immunoprecipitation are presented, which permits the identification of protein-DNA interactions in pull-down assays using specific antibodies against DNA-binding proteins.