Mouse Cre-LoxP system: general principles to determine tissue …
网页2018年12月31日 · A various tissue-specific Cre-driver mouse lines have been generated to date, and new Cre lines are still being developed. This review provides a brief overview of Cre- loxP system and a few commonly used promoters for expression of …
Orthogonal LoxPsym sites allow multiplexed site-specific ... - Nature
网页2024年2月7日 · The LoxP site is a 34 bp sequence comprising two 13 bp inverted repeats that flank a directional 8 bp spacer 42. Two Cre recombinase enzymes bind as a dimer to the inverted repeats of a LoxP...
LoxP Site - an overview | ScienceDirect Topics
网页In conceptualizing the targeting construct, the restriction enzyme sites should be designed not only to identify the appropriate targeting event but also to ensure the distal LoxP site is retained. The LoxP site closest to the selection marker is most often retained, but the distal LoxP site may be lost, especially if it is located several kb ...
Cre recombinase activity is inhibited in vivo but not ex vivo by a ...
网页In vivo, we found that a single T to C mutation at position 4 of the central spacer region in the distal (3') loxP site, completely inhibited the recombination reaction in two conditional mouse models.
Sequence of the loxP site determines the order of strand ... - PubMed
网页2003年2月14日 · We have confirmed this strand bias for an intermolecular recombination reaction that used wild-type loxP sites and Cre protein. We have examined the sequence determinants for this strand preference by selectively mutating the two asymmetric scissile base-pairs in the lox site (those immediately adjacent to the sites of cleavage by Cre).
Preferential synapsis of loxP sites drives ordered strand
网页The bacteriophage P1 Cre recombinase catalyzes site-specific recombination between 34-base-pair loxP sequences in a variety of topological contexts. This reaction is widely used to manipulate DNA molecules in applications ranging from benchtop cloning to genome modifications in transgenic animals.
Cre recombinase activity is inhibited in vivo but not ex vivo by a ...
网页2015年9月1日 · In vivo, we found that a single T to C mutation at position 4 of the central spacer region in the distal (3′) loxP site, completely inhibited the recombination reaction in two conditional mouse models.
Cre recombinase activity is inhibited in vivo but not ex vivo by a ...
网页2015年9月18日 · In vivo, we found that a single T to C mutation at position 4 of the central spacer region in the distal (3') loxP site, completely inhibited the recombination reaction …
Cre Recombinase Activity Is Inhibited In Vivo but Not Ex Vivo by a ...
网页2015年9月1日 · Sequencing chromatograms show the sequence of the distal (3′) loxP site and location of the mutation T > C at position 4 of the asymmetric central spacer region (underlined). This mutation...
Unidirectional Cre‐mediated genetic inversion in mice using …
网页2003年11月15日 · Site‐directed mutagenesis yielded a pair of asymmetric loxP sites ( lox66 and lox71) that display a favorable forward reaction equilibrium. Here, we demonstrate that lox66 / lox71 mediates efficient and predominantly unidirectional inversion of a switch substrate targeted to the mouse genome in combination with either inducible or cell type ...
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