Excited-State Dynamics of Proflavine after Intercalation into DNA ...
网页2022年11月23日 · In this paper, we studied the excited-state dynamics of proflavine and PF-DNA, in detail. With the help of both steady-state and time-resolved spectroscopy, the fluorescence quenching in PF-DNA is found to be a static-quenching mechanism. Both emissive states in proflavine exhibit one-order-of-magnitude smaller excited-state lifetimes in …
A biophysical investigation on the binding of proflavine with …
网页2016年12月1日 · Proflavine caused significant quenching of the intrinsic fluorescence of hemoglobin. • The quenching was due to complexation between proflavine and hemoglobin. • Proflavine induced significant secondary structural changes in hemoglobin. • DSC studies showed that the binding reaction caused destabilization of the protein.
Proflavine an acridine DNA intercalating agent and strong …
网页2017年12月1日 · Using 2′-deoxynucleotide-proflavine model systems, only guanosine-5′-monophosphate gives rise to a substantial quenching of the fluorescence. In contrast, all of the other nucleotides slightly enhanced the fluorescence of proflavine.
Excited-State Dynamics of Proflavine after Intercalation into
网页2022年11月23日 · The fluorescence-quenching mechanism of proflavine into DNA duplex and the excited-state deactivation mechanism were proposed, based on the spectroscopy data.
Photo-activated proflavine degrades protein and impairs enzyme …
网页2021年12月21日 · Free tryptophan quenches fluorescence by 63 percent when combined with proflavine or proflavine plus Cu(II) (Fig. 7 A). This demonstrates that BSA is one of the restricted proteins to which proflavine can …
Molecular Mechanism of Direct Proflavine–DNA Intercalation: …
网页With appropriate configurational restraint (to prevent dissociation) in all-atom metadynamics simulations, we capture the free energy surface of direct intercalation from minor groove-bound state for the first time using an anticancer agent proflavine.
Ultrafast spectroscopy study of DNA photophysics after proflavine ...
网页2024年3月25日 · Proflavine (PF), an acridine DNA intercalating agent, has been widespread applied as an anti-microbial and topical antiseptic agent due to its ability to suppress DNA replication. On the other hand, various studies show that PF intercalation to DNA can increase photogenotoxicity and has potential chances to induce carcinomas of …
Environmentally Driven Symmetry Breaking Quenches Dual …
网页2024年4月22日 · A recent experimental study demonstrated that the proflavine molecule exhibits surprising dual fluorescence in the gas phase, which is suppressed in polar solvent environments.
Structure and dynamics of proflavine association around DNA
网页2016年4月21日 · However, the sub-millisecond timescale observed in fluorescence kinetic experiments is often attributed to the binding of proflavine outside of DNA. Here, we have performed molecular dynamics simulations with multiple proflavine molecules to study the structure and dynamics of the formation of the outside bound state of DNA at different ion ...
A biophysical investigation on the binding of proflavine with …
网页2016年12月1日 · Proflavine caused significant quenching of the intrinsic fluorescence of hemoglobin. The quenching was due to complexation between proflavine and hemoglobin. Proflavine induced significant secondary structural changes in hemoglobin.