RNA-Seq - Wikipedia
网页RNA-Seq (named as an abbreviation of RNA sequencing) is a technique that uses next-generation sequencing to reveal the presence and quantity of RNA molecules in a biological sample, providing a snapshot of gene expression in the sample, also known as transcriptome. [ 2 ][ 3 ]
RNA Sequencing | RNA-Seq methods & workflows - Illumina
网页RNA-Seq with next-generation sequencing (NGS) is increasingly the method of choice for scientists studying the transcriptome. Covers an extremely broad dynamic range. Provides sensitive, accurate measurement of gene expression. Captures both known and novel features; does not require predesigned probes.
RNA Sequencing and Analysis - PMC - National Center for …
网页In addition to polyadenylated messenger RNA (mRNA) transcripts, RNA-Seq can be applied to investigate different populations of RNA, including total RNA, pre-mRNA, and noncoding RNA, such as microRNA and long ncRNA. This article provides an introduction to RNA-Seq methods, including applications, experimental design, and technical challenges.
A Beginner’s Guide to Analysis of RNA Sequencing Data
网页2017年12月14日 · In the present review, we provide a simplified overview that describes some basic, established methods for RNA-seq analysis and demonstrate how some important dataset characteristics, such as group size and intragroup variability, can affect downstream analysis.
RNA-Seq methods for transcriptome analysis - PubMed
网页In this study, we review current RNA-Seq methods for general analysis of gene expression and several specific applications, including isoform and gene fusion detection, digital gene expression profiling, targeted sequencing and single-cell analysis.
RNA sequencing: the teenage years | Nature Reviews Genetics
网页2019年7月24日 · This Review discusses advances in RNA-sequencing technologies and methods over the past decade and outlines adaptations that are enabling a fuller understanding of RNA biology, from when...
RNA sequencing | Nature Methods
网页2024年6月7日 · Here, we discuss the importance of long-read sequencing in interpreting the variables along RNA molecules, such as polyadenylation sites, transcription start sites, splice sites and other RNA...
RNA-Seq: Basics, Applications and Protocol - Technology Networks
网页2018年4月6日 · RNA-seq (RNA-sequencing) is a technique that can examine the quantity and sequences of RNA in a sample using next-generation sequencing (NGS). It analyzes the transcriptome, indicating which of the genes encoded in our DNA are turned on or off and to what extent.
RNA-Seq methods for transcriptome analysis - National Center …
网页RNA-Seq is now the method of choice to study gene expression and identify novel RNA species. Compared to DNA microarray-based methods, RNA-Seq offers less background noise and a greater dynamic range for detection. Most importantly, RNA-Seq directly reveals sequence identity, crucial for analysis of unknown genes and novel transcript isoforms.
RNA-Seq: a revolutionary tool for transcriptomics - Nature
网页RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and...
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