This technical advancement has initiated the trend of sequencing multiple samples in different conditions or environments to explore the similarities and dissimilarities of the microbial communities.

Technologies to mine the microbiome For decades, sequence-based bacterial analysis relied on genes encoding the 16S ribosomal RNA (rRNA). 16S rRNA genes consist of highly conserved nucleotide ...

The rRNA structures also provide binding sites for ribosomal proteins, which help stabilize the overall structure of ... analyses and the identification of microorganisms in environmental samples ...

2 However, although RNA-seq is becoming more accessible and prevalent, it comes with technical obstacles that can prevent scientists from obtaining the high-quality data they need. RNA is more subject ...

We further investigated the affiliation of different ecotypes with distinct small subunit ribosomal ribonucleic acid (SSU rRNA) genotypes, or molecular operational taxonomic units. These regional ...

In a healthy human adult, bacterial cells outnumber human cells, but the identity and degree of diversity of these bacteria in a single individual, their variability from person to person, and their ...

Next-generation sequencing (often noted as NGS), describes the high-throughput technologies that can now determine the sequence of a given nucleic acid strand, such as DNA or RNA, in a matter of hours ...

The MiSeq desktop sequencer offers low throughput flowcells and long read lengths. It is best for small whole-genome sequencing, targeted gene sequencing, metagenomics, and CRISPR validation. The ...

The researchers employed advanced techniques to analyze the bacterial DNA from the fecal samples, focusing on the 16S ribosomal RNA ... Additionally, the study’s reliance on 16S ribosomal RNA ...

Our group has two distinct functions: 1) We are a core facility providing DNA sequencing service (next-generation (AVITI and Illumina) and third-generation sequencing (PacBio, ONT) and Sanger).

Non-Watson-Crick base pairs can play important roles in RNA secondary structure, protein-nucleic acid interactions, and genetic recoding events. The Role of Base Pairs in Genetics Base pairs play a ...