Autofluorescence - Wikipedia
网页Autofluorescence is the natural emission of light by biological structures such as mitochondria and lysosomes when they have absorbed light, and is used to distinguish the light originating from artificially added fluorescent markers (fluorophores).
Autofluorescence Spectroscopy and Imaging: A Tool for …
网页2014年10月10日 · Native fluorescence, or autofluorescence (AF), consists in the emission of light in the UV-visible, near-IR spectral range when biological substrates are excited with light at suitable wavelength.
网页The best ways to address the issue of autofluorescence in order of preference: 1. Avoid it - not always possible. 2. Try to filter it out during image acquisition - difficult due to the broad emission spectrum. 3. Chemically remove it - can also reduce “real” signal.
A Biological Breakdown of Autofluorescence: Why Your Samples …
网页2023年3月30日 · Biology Is Brighter Than You Think―Defining Autofluorescence. Autofluorescence, the natural emission of light by biological structures, is a widely occurring phenomenon in cells and tissues. It’s caused by endogenous molecular components exhibiting the ability to fluoresce.
Autofluorescence - an overview | ScienceDirect Topics
网页Autofluorescence is the emission of fluorescent light from ocular structures in the absence of sodium fluorescein. Conditions that cause autofluorescence are optic nerve head drusen and astrocytic hamartoma ( Fig. 1.32 ).
Autofluorescence: Biological functions and technical applications ...
网页2015年7月1日 · The term “autofluorescence” is used to distinguish the fluorescence emitted by endogenous fluorophores in biological samples from that generated by the use of exogenous fluorescent dyes (not naturally present in a tissue) or genetically engineered fluorophores (e.g., green fluorescent protein).
Cell and tissue autofluorescence research and ... - ScienceDirect
网页2005年1月1日 · The term “autofluorescence” is used to distinguish the intrinsic fluorescence of cells and tissues from the fluorescence obtained by treating specimens with exogenous fluorescent markers that binds cell and tissue structures.
Demystifying autofluorescence with excitation-scanning …
网页The reduced acquisition time and increased signal-to-noise ratio allow for separation of significantly more fluorophores than previously possible. Here, we propose to utilize excitation-scanning of autofluorescence to examine tissues and diagnose pathologies.
Recent Advances and the Potential for Clinical Use of Autofluorescence …
网页2020年4月30日 · Endogenous fluorophores exist in human tissue and emit light upon excitation by a suitable wavelength, a phenomenon called autofluorescence (AF). AF is detectable in vitro and in vivo in real time without specific exogenous labeling.
Strong increase in the autofluorescence of cells signals
网页2018年8月14日 · Therefore, cellular autofluorescence (AF) is expected to vary with the metabolic states of cells. We examined how exposure to the different stressors changes the AF of Escherichia coli cells.
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