Distinct features of H3K4me3 and H3K27me3 chromatin domains …
网页2016年6月15日 · Here, by using a small-scale chromatin immunoprecipitation followed by sequencing (ChIP–seq) method 3, we map the genome-wide profiles of histone H3 lysine 4 trimethylation (H3K4me3) and...
H3K4me3 regulates RNA polymerase II promoter-proximal pause …
网页2023年3月1日 · These three proteins were also found to be preferentially enriched in the H3K4me3 ChIP–MS data, when compared with ChIP–MS data from other heterochromatin or non-promoter specific histone ...
Coordinated regulation of cellular identity–associated H3K4me3 …
网页ChIP-seq [chromatin immunoprecipitation (ChIP) followed by high-throughput sequencing] analyses exhibited similar H3K4me3 levels in wild-type (WT) and Set1BKO ESCs . In addition, the expressions of key pluripotency factors Sox2 , Nanog , and Oct4 remain invariable when comparing WT and Set1B KO cells (fig. S1E).
The dynamic broad epigenetic (H3K4me3, H3K27ac) domain as a …
网页2021年7月8日 · A meta-analysis of H3K4me3 ChIP-seq data across > 20 different cell and tissue types in mice and humans, revealed a subset of genes marked by a broad H3K4me3 domain spanning the TSS. In identifying genes with the broad H3K4me3 domain, typically ChIP-seq peaks are called using the broad-peak function in MACS2 [ 9 , 42 , 43 , 44 ].
易基因｜表观发育：ChIP-seq揭示精子H3K4me3可传递到胚胎并 …
网页2022年2月17日 · 本研究利用染色质免疫共沉淀测序 (ChIP-seq) 和转录组测序（RNA-seq）技术，表明精子 h3k4me3可能作为一种代谢传感器，通过胞衣将父方的饮食与后代的表型联系起来。
易基因：人精子H3K4me3 ChIP-seq和DNA甲基化WGBS揭示与生 …
网页2021年7月20日 · H3K4me3 peaks和DNA甲基化之间的重叠显示在基因组浏览器快照中，显示了HOXA发育基因簇（A）的ChIP-seq轨迹(蓝色)、MACS2 calling的H3K4me3 peaks(深蓝色)、WGBS轨迹(黑色)、CpG岛位点轨迹(红色，UCSC)和GC百分比热图(蓝色，低GC %;白色，50% GC;红色，高GC %)，染色质修饰蛋白ARID5B (B ...
Nature：王华等揭示H3K4me3在转录调控中的具体作用_澎湃号·湃 …
网页2023年3月1日，美国纪念斯隆-凯瑟琳癌症中心 Kristian Helin 研究组在 Nature 发表了题为：H3K4me3 regulates RNA polymerase II promoter-proximal pause-release 的研究长文，对这一长期困扰学界的问题进行了回答。. 该研究发现，H3K4me3在细胞体内是通过转录暂停-释放过程来调节基因 ...
Allelic reprogramming of the histone modification H3K4me3 in …
网页2016年9月14日 · The strong correlation between the presence of PMDs and ncH3K4me3 indicates that the reprogramming of histone modifications and DNA methylation is inherently linked. Using a highly sensitive ChIP...
Whole-genome sequencing of H3K4me3 and DNA methylation in …
网页Lambrot et al. use H3K4me3 ChIP-seq and WGBS to profile the sperm epigenome of Canadian men. Deep sequencing reveals regions enriched for H3K4me3 that overlap with DNA methylation, suggesting a functional relationship in sperm.
H3K4me3 recognition by the COMPASS complex facilitates the
网页2022年5月6日 · During DNA replication, parental H3-H4 marked by H3K4me3 are transferred almost equally onto leading and lagging strands of DNA replication forks. Mutations in replicative helicase subunit, Mcm2 (Mcm2-3A), and leading strand DNA polymerase subunit, Dpb3 (dpb3 ∆), result in asymmetric distributions of H3K4me3 at replicating DNA strands ...
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