What is Anther Culture? History, Methods and Protocol - Biology …
网页Anther culture is a type of tissue culture technique, where a part of a plant (anther) gives rise to an androgenic haploid plant when cultured on a nutrient medium under optimal conditions. The nutrient media provide all the necessary factors that are required for the growth of the cell.
Anther Culture - an overview | ScienceDirect Topics
网页This is a technique by which immature pollen is made to divide and grow into tissue (either callus or embryonic tissue), primarily to produce haploids (plants with an N chromosome number) known to be anther culture. Haploid production through anther culture has been referred to as androgenesis.
Anther Culture - an overview | ScienceDirect Topics
网页Anther culture is a supplementary in vitro technique for breeding work in many plant species (Jain et al., 1996). Anthers cultured in vitro develop into plantlets either by direct microspore embryogenesis or by organogenesis, providing valuable breeding material, such as haploid plants, or homozygous diploid lines.
Anther Culture: A High-Throughput Method for Haploid Plant …
网页2023年4月25日 · Anther culture is a type of tissue culture technique used to produce haploids and dihaploids. It is simpler than the pollen culture technique. It uses microspores or anthers for plant regeneration. The first successful report of anther culture was published in the 1970s by Guha and Maheshwari on.
Anther culture for haploid and doubled haploid production
网页2010年11月12日 · There are several available methods to obtain haploids and DHs, of which in vitro anther or isolated microspore culture are the most effective and widely used. This review article deals with the current status of knowledge on the production of haploids and DHs through pollen embryogenesis and, in particular, anther culture.
Anther culture for haploid plant production - ScienceDirect
网页2022年1月1日 · Haploid production from the anther or pollen culture is known as androgenesis. Haploid plants are the sporophytes that possess gametic chromosome number. Haploids have now become a valuable source for the screening of desired traits and mutation breeding.
Anther culture in rice proportionally rescues microspores …
网页2018年11月17日 · Methods that maintain immature microspores as living cells can rescue pollen aborted between meiosis and fertilization and enable genetic studies of gametophytic characteristics. Anther culture (AC) is a technique in which plants are regenerated from microspores via callus formation in in vitro culture [1, 2]. Callus induced from microspores ...
Anther culture of Anthurium : a review - Springer
网页2015年8月5日 · The use of anther culture to produce haploid plants is a useful breeding technique for Anthurium, but has only seen limited applications thus far. This review describes the advances achieved thus far, and provides practical tips and protocols that would allow researchers new to the field, or already experienced Anthurium tissue culture ...
BASIC PRINCIPLES AND RECENT ADVANCES IN ANTHER/POLLEN CULTURE …
网页2017年12月15日 · Anther/microspore culture will not only yield pure haploid plants but also produces the undesired heterozygous plantlets (diploid, triploid, tetraploid, and so on) plants.
Improved Anther Culture Media for Enhanced Callus Formation and Plant ...
网页2021年4月22日 · In this study, two improved anther culture media were evaluated based on their ability to enhance the callus formation and green plant regeneration of five rice genotypes that belonged to different subspecies, which have …